The 3’–OH end is called a primer.

A sequence of RNA is synthesized on the template, so that the free 3’–OH end of the RNA chain is extended by DNA Pol and This is commonly used in replication of cellular DNA and by some viruses.

In case of reteroviruses, A preformed RNA (often a tRNA) pairs with the template, allowing its 3’–OH end to be used to prime DNA synthesis.

TLS Online TPP Program

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#Unit 13. Methods in Biology

DIC microscopy is used for visualizing extremely small details and thick objects.

TLS Online TPP Program

#Id: 4478

#Unit 13. Methods in Biology

DIC microscopy easily defines the outlines of large organelles, such as the nucleus and vacuole.

TLS Online TPP Program

#Id: 4479

#Unit 13. Methods in Biology

Formaldehyde a common fixative, crosslinks amino groups on adjacent molecules

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#Unit 13. Methods in Biology

Histological samples are often stained with hematoxylin and eosin (“H&E stain”).

TLS Online TPP Program

#Id: 4481

#Unit 13. Methods in Biology

Hematoxylin binds to basic amino acids (lysine and arginine).

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#Id: 4482

#Unit 13. Methods in Biology

Eosin binds to acidic molecules (DNA and side chains of aspartate and glutamate).