TLS Online TPP Program

#Id: 8203

#Unit 2. Cellular Organization

An H+ ATPase in the membrane pumps H+ into the lysosome, maintaining its lumen at an acidic pH.

TLS Online TPP Program

#Id: 729

#Unit 3. Fundamental Processes

DnaA is the E. coli licensing factor that must be made anew for each round of replication.

TLS Online TPP Program

#Id: 2933

#Unit 3. Fundamental Processes

The E. coli oriC is fully methylated at the beginning of replication.

TLS Online TPP Program

#Id: 6289

#Unit 3. Fundamental Processes

This consensus sequence was derived by aligning 300 sequences known to function as s70 promoters and ascertaining the most common base found at each position in the –35 and –10 hexamers. That nucleotide is then chosen as the nucleotide of choice at that position in the consensus; its relative frequency and the frequencies with which the other three nucleotides occur at each position are portrayed in the graph. 

Note that there is no significant consensus among the 17–19 nucleotides that lie in the region between –35 and –10.

TLS Online TPP Program

#Id: 2612

#Unit 13. Methods in Biology

Our on-going research indicates that RLMRACE, PPM-RACE, and qRT-PCR are very effective in the verification of sequences of miRNA targets obtained by Degradome sequencing.