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TLS Online TPP Program

#Id: 639

The +vely charged acridine dyes or acridine orange are potent mutagens that induce frameshift mutations by Intercalation.

#Unit 3. Fundamental Processes #Eukaryotic Chromosome Architecture #Part B Pointers
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TLS Online TPP Program

#Id: 7148

#Unit 6. System Physiology – Plant

In plants, mechanical damage or insect feeding can cause rapid and transient accumulation of JA and JA-Ile at the site of injury, thereby activating the expression of defense genes surrounding the wound and producing a local defense response
TLS Online TPP Program

#Id: 904

#Unit 13. Methods in Biology

Forward light scattering detect shape and side scattering detects granularity and complexity of the cell
TLS Online TPP Program

#Id: 9332

#Unit 4. Cell Communication and Cell Signaling

In bright light, little transducin is found in the outer segment, and abundant arrestin is found there; in this condition,vision is relatively insensitive to small changes in light.
TLS Online TPP Program

#Id: 2775

#Unit 13. Methods in Biology

Methylation interference assay Dimethyl sulphate (DMS) methylates purines on DNA strand. Methylate DNA prior to adding protein. Protein binding is prevented at methylated sites. After treatment and removal of protein of interest, treat with piperidine or NaOH which cuts strand at modified bases.
TLS Online TPP Program

#Id: 8773

#Unit 1. Molecules and their Interaction Relevant to Biology

Techniques for Measuring Stability

Absorbance (e.g. Trp, Tyr) 

Fluorescence (Trp)-difference in emission max & intensity.

CD (far or near UV) - (2o or 3o) 

NMR 

DSC (calorimetry) 

Urea gradient gels - difference in the migrating rates between F and U.

Catalytic activity 

Chromophoric or fluorophoric probes/FRET