Phosphorylation of a critical threonine residue in the activation loop, Thr-160, allowing substrates to access the active site of the CDK

TLS Online TPP Program

#Id: 3123

#Unit 3. Fundamental Processes

Replication generates hemimethylated DNA, which cannot initiate replication

TLS Online TPP Program

#Id: 3124

#Unit 3. Fundamental Processes

There is a 13-minute delay before the GATC/CTAG repeats are remethylated. 

TLS Online TPP Program

#Id: 3126

#Unit 3. Fundamental Processes

oriC contains binding sites for DnaA: dnaA-boxes.DnaA is an ATP-bindingprotein and its binding to DNA is affected depending on whether ATP, ADP, or no nucleotide is bound.

TLS Online TPP Program

#Id: 3127

#Unit 3. Fundamental Processes

One mechanism by which the activity of the replication origin is controlled is DNA methylation. 

TLS Online TPP Program

#Id: 3128

#Unit 3. Fundamental Processes

The E. coli oriC contains 11 copies of the sequence GATC/CTAG which is a target for methylation at the N6 position of adenine by the Dam methylase enzyme.

TLS Online TPP Program

#Id: 3129

#Unit 3. Fundamental Processes

Before replication, the palindromic target site is methylated on the adenines of each strand.