Phosphorylation of a critical threonine residue in the activation loop, Thr-160, allowing substrates to access the active site of the CDK

TLS Online TPP Program

#Id: 8858

#Unit 2. Cellular Organization

Cohesin-mediated cohesion between sister chromatids is established by a two-step process:

1.Loading and unloading of cohesins

2. Stabilization of cohesins

TLS Online TPP Program

#Id: 8859

#Unit 2. Cellular Organization

During G1, 

cohesins associate with chromosomes by cohesin loading factors Scc2 and Scc4 and unloaded continuously by a cohesin associated complex Pds5 and Wap1 proteins. 

TLS Online TPP Program

#Id: 8860

#Unit 2. Cellular Organization

During S phase

DNA-bound G1 cohesins Convert into cohesive complexes by cohesin acetyl transferases (CoATs)

which acetylate Smc3 subunit & prevents unloading of cohesins by Pds5-Wapl, thereby stabilizing the cohesins on chromosomes.

TLS Online TPP Program

#Id: 8861

#Unit 2. Cellular Organization

TLS Online TPP Program

#Id: 8862

#Unit 2. Cellular Organization

In vertebrates, stabilization of cohesins requires the cohesin-associated factor sororin.

TLS Online TPP Program

#Id: 8863

#Unit 2. Cellular Organization

Defects of cohesin’s gene expression regulation function are the cause of a group of diseases collectively called cohesinopathies.