Molecular genetic techniques are used to make a cDNA encoding a recombinant protein to which is fused a short sequence of amino acids called an epitope tagging.

TLS Online TPP Program

#Id: 8531

#Unit 2. Cellular Organization

Dynamic instability 

Rapid interconversion between a growing and shrinking state, at a uniform free tubulin concentration

TLS Online TPP Program

#Id: 8532

#Unit 2. Cellular Organization

Catastrophe 

The change from growth to shrinkage

TLS Online TPP Program

#Id: 8533

#Unit 2. Cellular Organization

Rescue 

The change from shrinkage to growth

TLS Online TPP Program

#Id: 8534

#Unit 2. Cellular Organization

γ-tubulin ring complex (γ-TuRC)

nucleates assembly of a microtubule by forming a template corresponding to the (−) end. 

TLS Online TPP Program

#Id: 8535

#Unit 2. Cellular Organization

Augmin

an 8-subunit protein complex that binds to sites along the microtubule and recruits γ-TuRC, which nucleates a new microtubule to form a microtubule branch.

TLS Online TPP Program

#Id: 8536

#Unit 2. Cellular Organization

Plant cells rely extensively on augmin-dependent microtubule branching nucleation to organize the microtubule cytoskeleton because they lack centrosomes.