Molecular genetic techniques are used to make a cDNA encoding a recombinant protein to which is fused a short sequence of amino acids called an epitope tagging.

TLS Online TPP Program

#Id: 1346

#Unit 4. Cell Communication and Cell Signaling

Post-translational modifications of the CAAX motifs determine the subcellular localization and correct biological function of Ras proteins.

TLS Online TPP Program

#Id: 1347

#Unit 4. Cell Communication and Cell Signaling

SOS – Ras GEF

NF1 - Ras GAP

TLS Online TPP Program

#Id: 1348

#Unit 4. Cell Communication and Cell Signaling

RasD or RasONC  Replacement of the normal glycine 12 with any other amino acid (except proline) “locks” Ras in the active GTP-bound state.

TLS Online TPP Program

#Id: 1349

#Unit 4. Cell Communication and Cell Signaling

RAF (also known as MAPKKK),  A ser/thr protein kinase

TLS Online TPP Program

#Id: 1350

#Unit 4. Cell Communication and Cell Signaling

KSR (Kinase suppressor of Ras) is a pseudogene homolog of RAF Involve in the activation of RAF

TLS Online TPP Program

#Id: 1351

#Unit 4. Cell Communication and Cell Signaling

CNK serves as a molecular scaffold platform for KSR-mediated RAF activation