Nurturing Life Sciences
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#Unit 3. Fundamental Processes
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RNA Polymerase SDS page shows two very large subunits: beta (b) and beta-prime (b’), with molecular masses of 150 and 160 kD, respectively.
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The holoenzyme could transcribe intact phage T4 DNA in vitro quite actively, the core enzyme had little ability to do this.
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Hinkle and Chamberlin experiment showed that the polymerase holoenzyme binds much more tightly to the T7 DNA than does the core enzyme.
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Hinkle and Chamberlin experiment also showed that the melted DNA has higher affinity for holoenzyme
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A promoter is defined by the presence of short consensus sequences at specific locations.