The group I and group II introns have the remarkable ability to excise themselves from an RNA. This is called autosplicing, or self splicing. 

Group I introns are more common than group II introns.

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#Id: 919

#Unit 13. Methods in Biology

Other dyes that bind to DNA and allow for similar types of cell cycle analysis include DAPI, Hoechst 33342, and 7-aminoactinomycin D (AAD).

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#Id: 920

#Unit 13. Methods in Biology

Carboxyfluorescein succinimidyl ester (CFSE) is more correctly named carboxyfluorescein diacetate succinimidyl ester (CFDASE) very commonly used to detect state of cell 

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#Id: 921

#Unit 13. Methods in Biology

(CFSE) or carboxyfluorescein diacetate succinimidyl ester (CFDASE) is nontoxic to cells hence retained till many cycles

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#Id: 922

#Unit 13. Methods in Biology

CFSE labeling amount of fluorescence emitted is cut in half each time the cell divides.

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#Id: 923

#Unit 13. Methods in Biology

The 51Cr Release Assay is used to measure Cell death not apoptosis

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#Id: 924

#Unit 13. Methods in Biology

Normal cells treated with sodium chromate allowed to absorb and induced to death signals as T-CTLs Death of the target cells is indicated by the release of 51Cr into the supernatant