The mechanism of pre-tRNA splicing differs in three fundamental ways from the mechanisms used by self-splicing introns and spliceosomes.

First, splicing of pre-tRNAs is catalyzed by proteins, not by RNAs. Second, a pre-tRNA intron is excised in one step thatentails simultaneous cleavage at both ends of the intron. Finally, hydrolysis of GTP and ATP is required to join the two tRNA halves generated by cleavage on either side of the intron.

TLS Online TPP Program

#Id: 2469

#Unit 13. Methods in Biology

Phenomenon of Electroendosmosis is largely applicable in Free Solution & Capillary.

TLS Online TPP Program

#Id: 5431

#Unit 7. System Physiology – Animal

Two types of potentials are produced:

Nonpropagated potentials (local), depending on their location, synaptic, generator, or electrotonic potentials

Propagated potentials, the action potentials (or nerve impulses)

TLS Online TPP Program

#Id: 8558

#Unit 1. Molecules and their Interaction Relevant to Biology

Similarly, if the inhibitor binds to a different site from the substrate and reduces the affinity of the enzyme for the substrate without altering the reaction characteristics of that substrate which does bind, then hyperbolic competitive inhibition results

TLS Online TPP Program

#Id: 9076

#Unit 4. Cell Communication and Cell Signaling

A G protein– coupled receptor kinase phosphorylates serine and threonine residues in the

cytoplasmic segments of activated GPCRs

TLS Online TPP Program

#Id: 2755

#Unit 13. Methods in Biology

Linker and/or adapter molecules can be joined to the cut ends. Linkers are short, chemically synthesized, double-stranded oligonucleotides, which contain within them one or more restriction endonuclease sites, e.g., linker 5'CCGAATTCGG (only one strand of the linker is shown here) contains one EcoRI site.