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#Id: 6704

The mechanism of pre-tRNA splicing differs in three fundamental ways from the mechanisms used by self-splicing introns and spliceosomes.
First, splicing of pre-tRNAs is catalyzed by proteins, not by RNAs. Second, a pre-tRNA intron is excised in one step thatentails simultaneous cleavage at both ends of the intron. Finally, hydrolysis of GTP and ATP is required to join the two tRNA halves generated by cleavage on either side of the intron.

#Unit 3. Fundamental Processes #Capping, Polyadenylation, Splicing and Alternative splicing #Part B Pointers

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#Unit 13. Methods in Biology

Enzyme-linked immunosorbent assay, commonly known as ELISA (or EIA), is similar in principle to RIA but depends on an enzyme rather than a radioactive label.

TLS Online TPP Program

#Id: 4679

#Unit 13. Methods in Biology

Antigen can be detected or measured by a sandwich ELISA .In this technique, the antibody (rather than the antigen) is immobilized on a microtiter well.

A sample containing antigen is added and allowed to react with the immobilized antibody. After the well is washed, a second enzyme- linked antibody specific for a different epitope on the antigen is added and allowed to react with the bound antigen

After any free second antibody is removed by washing, substrate is added, and the colored reaction product is measured.

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#Id: 4680

#Unit 13. Methods in Biology

What are the principles in flow cytometry?

Light scattered by a laser or arc lamp

Specific fluorescence detection

Hydrodynamically focused stream of particles

Electrostatic particle separation for sorting

Multivariate data analysis capability

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#Id: 4681