crRNA and Cascade are then directed to and cleave the target DNA with the help of Cas3 in E.coli

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#Id: 878

#Unit 13. Methods in Biology

The efficiency of FRET is inversely proportional to the sixth power of the distance between donor and acceptor.

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#Id: 879

#Unit 13. Methods in Biology

Short tandem repeats (STRs) are short repeated sequences of DNA (1–10 bp) that account for approximately  3% of the human genome.

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#Id: 880

#Unit 13. Methods in Biology

AAAAAAAAAAAAAAA (a 1-base repeat) and CACACACACACACACACACACA (a 2-basen repeating), CAGCAGCAGCAGCAGCAG (a 3 base repeating) are most common.

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#Id: 881

#Unit 13. Methods in Biology

Most STRs are found in the noncoding regions, while only about 8% locate in the coding regions.

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#Id: 882

#Unit 13. Methods in Biology

The genetic diseases caused by trinucleotide repeat SSRs within genes. Huntington disease (HD) is one of the approximately 20 such trinucleotide repeat diseases in humans. HD is transmitted as an autosomal dominant mutation.

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#Id: 883

#Unit 13. Methods in Biology

In humans, chromosome 19 has the highest density of STRs. Disease associated with SSR are called as Repeat expansion diseases.