The ZnA region of the protein assists in the initial RAG1 binding to active chromatin via its ability to interact with histone H3.

TLS Online TPP Program

#Id: 2894

#Unit 3. Fundamental Processes

Dna A has intrinsic ATPase activity that hydrolyzes ATP to ADP and thus inactivates itself when the initiation stage ends.

TLS Online TPP Program

#Id: 2895

#Unit 3. Fundamental Processes

This ATPase activity of Dna A is stimulated by membrane phospholipids and single-stranded DNA.

TLS Online TPP Program

#Id: 2897

#Unit 3. Fundamental Processes

The origin of the replication region remains attached to the membrane for about one-third of the cell cycle as another part of the mechanism to prevent reinitiation.

TLS Online TPP Program

#Id: 2899

#Unit 3. Fundamental Processes

Opening oriC involves action at two types of sequence in the origin: 9-bp and 13 -bp repeats.

TLS Online TPP Program

#Id: 2903

#Unit 3. Fundamental Processes

The four 9-bp consensus sequences on the right side of oriC provide the initial binding sites for DnaA-ATP in an extended multimeric state promoted by the accessory protein DiaA, which stimulates cooperative binding of DnaA.

TLS Online TPP Program

#Id: 2904

#Unit 3. Fundamental Processes

DnaA-ATP binds cooperatively to form a helical central core around which oriC DNA is wrapped.