A molecule is the smallest part of a substance that has the physical and chemical properties of that substance. In some respects, a molecule is similar to an atom. A molecule, however, is composed of more than one atom.

TLS Online TPP Program

#Id: 918

#Unit 13. Methods in Biology

Propidium iodide-labeled cells in order as

1. Apoptotic cells

2. G1/G0

3. S

4. G2/M  

TLS Online TPP Program

#Id: 919

#Unit 13. Methods in Biology

Other dyes that bind to DNA and allow for similar types of cell cycle analysis include DAPI, Hoechst 33342, and 7-aminoactinomycin D (AAD).

TLS Online TPP Program

#Id: 920

#Unit 13. Methods in Biology

Carboxyfluorescein succinimidyl ester (CFSE) is more correctly named carboxyfluorescein diacetate succinimidyl ester (CFDASE) very commonly used to detect state of cell 

TLS Online TPP Program

#Id: 921

#Unit 13. Methods in Biology

(CFSE) or carboxyfluorescein diacetate succinimidyl ester (CFDASE) is nontoxic to cells hence retained till many cycles

TLS Online TPP Program

#Id: 922

#Unit 13. Methods in Biology

CFSE labeling amount of fluorescence emitted is cut in half each time the cell divides.

TLS Online TPP Program

#Id: 923

#Unit 13. Methods in Biology

The 51Cr Release Assay is used to measure Cell death not apoptosis