Isomers that have opposite configurations at one or more of the chiral centers but that are not mirror images of each other are called diastereomers

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#Id: 4596

#Unit 13. Methods in Biology

Diphenyltetrazolium bromide, a yellow tetrazole is reduced by metabolically active cells to form insoluble, purple formazan dye crystals detected at 570nm

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#Id: 4597

#Unit 13. Methods in Biology

The more metabolically active cells that are present in the culture, the more formazan will be generated, and so this assay provides a readout of the number of live cells as a function of time.

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#Id: 4598

#Unit 13. Methods in Biology

 The MTT assay can measure cell proliferation or cell death.

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#Id: 4599

#Unit 13. Methods in Biology

bromodeoxyuridine (BrdU) is rapidly phosphorylated and incorporated in its place into newly synthesized DNA hence BrdU is used to detect replicating cells.

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#Id: 4600

#Unit 13. Methods in Biology

BrdU can CAUSE light induced cell death

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#Id: 4601

#Unit 13. Methods in Biology

If cells that have incorporated a high level of BrdU are exposed to light, they will photolyse; this has been used to selectively kill newly dividing cells.