Hinkle and Chamberlin experiment also showed that the melted DNA has higher affinity for holoenzyme

TLS Online TPP Program

#Id: 3279

#Unit 3. Fundamental Processes

 In Escherichia coli, mismatches are detected by a dimer of the mismatch repair protein MutS.

TLS Online TPP Program

#Id: 3280

#Unit 3. Fundamental Processes

MutS embraces the mismatch-containing DNA, inducing a pronounced kink in the DNA and a conformational change in MutS itself.

TLS Online TPP Program

#Id: 3281

#Unit 3. Fundamental Processes

MutS has an ATPase activity that is required for mismatch repair, in Escherichia coli, mismatches are detected by a dimer of the mismatch repair protein MutS.

TLS Online TPP Program

#Id: 3282

#Unit 3. Fundamental Processes

In Escherichia coli,  the complex of MutS and the mismatch-containing DNA recruits MutL, a second protein component of the repair system. 

TLS Online TPP Program

#Id: 3283

#Unit 3. Fundamental Processes

In Escherichia coli MMR system, MutL, in turn, activates MutH, an enzyme that causes an incision or nick on one strand near the site of the mismatch.

TLS Online TPP Program

#Id: 3284

#Unit 3. Fundamental Processes

Nicking is followed by the action of a specific helicase (UvrD) and one of three exonucleases.