β-sheets shows a negative bands at 218nm and a positive one at 196nm.

Random coils has a positive band at 212nm and a negative one around 195nm.

TLS Online TPP Program

#Id: 3013

#Unit 3. Fundamental Processes

Helicase, It may function with a particular polarity, preferring single-stranded DNA with a 3 ' end (3'-5' helicase) or with a 5' end ( 5 '-3' helicase).

TLS Online TPP Program

#Id: 3014

#Unit 3. Fundamental Processes

E. coli SSB is a tetramer of 74 kD that binds single-stranded DNA cooperatively. The significance of the cooperative mode of binding.

TLS Online TPP Program

#Id: 3015

#Unit 3. Fundamental Processes

E. coli SSB- Note that this protein is not a DNA-unwinding protein; its function is to stabilize DNA that is already in the single-stranded condition

TLS Online TPP Program

#Id: 3016

#Unit 3. Fundamental Processes

ssb mutants have a quick stop phenotype, and are defective in repair and recombination as well as in replication. 

TLS Online TPP Program

#Id: 3017

#Unit 3. Fundamental Processes

All DNA polymerases require a 3'-0H priming end to initiate DNA synthesis.

TLS Online TPP Program

#Id: 3018

#Unit 3. Fundamental Processes

The priming end can be provided by an RNA primer, a nick in DNA, or a priming protein.