In mammalian cells, Rab5 protein (early endosomes) and its effector protein known as EEA1 (early endosome antigen 1)

#Id: 2277

#Unit 3. Fundamental Processes

Core enzyme without the s subunit could not transcribe viral DNA, yet had no problems with highly nicked calf thymus DNA

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#Unit 3. Fundamental Processes

With s subunit, the holoenzyme worked equally well on both types of DNA

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#Unit 3. Fundamental Processes

Consensus Sequences

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#Unit 3. Fundamental Processes

The carboxy-terminal domain of the a subunit (aCTD) recognizes the Up-element (where present), whereas s regions 2 and 4 recognize the –10 and –35 regions

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#Unit 3. Fundamental Processes

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#Unit 3. Fundamental Processes

Note that there is no significant consensus among the 17–19 nucleotides that lie in the region between –35 and –10.