The two ends of microfilament polymerize at different rates:

fast-growing end - plus end

slow-growing end - minus end

TLS Online TPP Program

#Id: 4588

#Unit 13. Methods in Biology

Flow cytometry or FACS is the sine qua non (without which, nothing) of the modern immunologist’s toolbox.

TLS Online TPP Program

#Id: 4589

#Unit 13. Methods in Biology

 Flow cytometry is based on light scattering and fluorescence 

TLS Online TPP Program

#Id: 4590

#Unit 13. Methods in Biology

Forward light scattering detect shape and side scattering detects granularity and complexity of the cell

TLS Online TPP Program

#Id: 4591

#Unit 13. Methods in Biology

Hydrodynamically focused stream separate cells in single file

TLS Online TPP Program

#Id: 4592

#Unit 13. Methods in Biology

2D Histogram

TLS Online TPP Program

#Id: 4593

#Unit 13. Methods in Biology

Flow cytometry is majorly used with fluorescence dyes and attached antibodies for cells and dyes.