Threading is a computational technique that attempts

to determine the unknown structure of a protein by ascertaining

whether it is consistent with a known protein structure.

TLS Online TPP Program

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#Unit 2. Cellular Organization

The Oc mutations are cis-acting/cis dominant, because they affect only the contiguous set of structural genes.

 

TLS Online TPP Program

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#Unit 2. Cellular Organization

The DNA-binding domain occupies residues 1–59. It contains two a-helices separated by a turn. This is a common DNA-binding motif known as the HTH (helix-turn-helix); the two a-helices fit into the major groove of DNA

TLS Online TPP Program

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#Unit 2. Cellular Organization

Promoter mutations are also cis-acting. If they prevent RNA polymerase from binding at P-lac, the structural genes are never transcribed.

TLS Online TPP Program

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#Unit 2. Cellular Organization

Mutants that cannot be expressed at all are called uninducible. Mutants that are continuously expressed are called constitutive mutants.

TLS Online TPP Program

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#Unit 2. Cellular Organization

“Cryptic mutants” of lac operon make b-galactosidase but still could not grow on lactose means they are essentially Y-

TLS Online TPP Program

#Id: 580

#Unit 2. Cellular Organization

Catabolite repression is exerted through a second messenger called cyclic AMP (cAMP) and the positive regulator protein called the catabolite repressor protein (CRP), a dimer.