Lamins B are post-translationally prenylated, which helps them associate with the inner nuclear envelope membrane.

TLS Online TPP Program

#Id: 2485

#Unit 13. Methods in Biology

Photopolymerization is an alternative method that can be used to polymerize acrylamide gels. Ammonium persulfate and TEMED are replaced by riboflavin and when the gel is poured it is placed in front of a bright light for 2–3 h.

TLS Online TPP Program

#Id: 731

#Unit 3. Fundamental Processes

In dam- E. coli If the plasmid is methylated, it undergoes a single round of replication, and if it is hemimethylated products accumulate

TLS Online TPP Program

#Id: 6289

#Unit 3. Fundamental Processes

This consensus sequence was derived by aligning 300 sequences known to function as s70 promoters and ascertaining the most common base found at each position in the –35 and –10 hexamers. That nucleotide is then chosen as the nucleotide of choice at that position in the consensus; its relative frequency and the frequencies with which the other three nucleotides occur at each position are portrayed in the graph. 

Note that there is no significant consensus among the 17–19 nucleotides that lie in the region between –35 and –10.

TLS Online TPP Program

#Id: 766

#Unit 3. Fundamental Processes

Two bases in the non-template strand of the –10 element (A11 and T7) flip out from their base-stacking interactions and instead insert into pockets within the sigma protein where they make more favorable interactions for OPC

TLS Online TPP Program

#Id: 3643

#Unit 3. Fundamental Processes

The Class II synthetases lack the foregoing sequences but have three other sequences in common.