Guanidinium ion and urea are chaotropic agents which disrupts hydrophobic core of proteins.

TLS Online TPP Program

#Id: 2750

#Unit 13. Methods in Biology

Amplified Fragment length Polymorphism (AFLP) shares some features of both RFLP and RAPD analyses.

TLS Online TPP Program

#Id: 2751

#Unit 13. Methods in Biology

AFLP uses restriction enzyme-digested genomic DNA as template for PCR amplification using primers that contain the restriction enzyme recognition sites plus a number of, usually 2-3, arbitrary nucleotides.

TLS Online TPP Program

#Id: 2752

#Unit 13. Methods in Biology

AFLPs are faster, less labour intensive and provide more information than RFLPs, and they are highly reproducible, which is a great advantage over RAPDs.

TLS Online TPP Program

#Id: 2753

#Unit 13. Methods in Biology

TLS Online TPP Program

#Id: 2754

#Unit 13. Methods in Biology

An AFLP is detected as a differential mobility of an amplified DNA fragment; AFLPs, therefore, behave as codominant markers during inheritance.

TLS Online TPP Program

#Id: 2755

#Unit 13. Methods in Biology

Linker and/or adapter molecules can be joined to the cut ends. Linkers are short, chemically synthesized, double-stranded oligonucleotides, which contain within them one or more restriction endonuclease sites, e.g., linker 5'CCGAATTCGG (only one strand of the linker is shown here) contains one EcoRI site.