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#Unit 3. Fundamental Processes
At the end of the cell cycle, ORC is bound to A–B1 elements of the origin. There is a change during G1 that results from the binding of Cdc6 and Cdt1 proteins to the ORC.
In yeast, Cdc6 is a highly unstable protein, with a half-life of 5 minutes. It is synthesized during G1 and typically binds to ORC between the exit from mitosis and late G1. Its rapid degradation means that no protein is available later in the cycle.