TLS Online TPP Program

#Id: 898

#Unit 13. Methods in Biology

Terminal transferase (TdT) Adds homopolymer tails to the 3’end of DNA.

TLS Online TPP Program

#Id: 899

#Unit 13. Methods in Biology

T4 DNA ligase Links 5-phosphate and 3-hydroxyl ends via phosphodiester bond.

TLS Online TPP Program

#Id: 900

#Unit 13. Methods in Biology

T4 polynucleotide kinase (PNK) Transfers terminal phosphate groups from ATP to 5-OH groups.

TLS Online TPP Program

#Id: 901

#Unit 13. Methods in Biology

Alkaline phosphatase Removes 5’ phosphates from DNA and RNA.

TLS Online TPP Program

#Id: 902

#Unit 13. Methods in Biology

Flow cytometry or FACS is the sine qua non (without which, nothing) of the modern immunologist’s toolbox.

TLS Online TPP Program

#Id: 903

#Unit 13. Methods in Biology

Flow cytometry is based on light scattering and fluorescence