Inbreeding coefficient (F)

Probability that an individual has two identical alleles for a given gene.

Inbreeding coefficients can range from 0 to 1.

#Id: 2778

#Unit 13. Methods in Biology

If the sample is hydrophobic Organic solvent is used, then to separat

Differing molecular size - Molecular exclusion system.

Weakly ionic or polar - Reversed-phase partition system.

Strongly ionic - Ion-exchange system or Reversed-phase partition system with lon-pairing is used.

#Id: 2779

#Unit 13. Methods in Biology

General structure and component of a chromatographic system

A stationary phase – typically an aqueous buffer with sufficient ionic strength to maintain a soluble protein.

A column -

An injector system: To deliver test samples to the top of the column in a reproducible manner.

A detector with data acquisition - To give a continuous record of the presence of the analytes in the eluate as it emerges from the column.

A fraction collector: For collecting the separated analytes for further biochemical studies.

#Id: 2780

#Unit 13. Methods in Biology

The basis of all forms of chromatography is the distribution or partition coefficient (Kd), which describes the way in which a compound (the analyte) distributes between two immiscible phases.

#Id: 2781

#Unit 13. Methods in Biology

A chromatogram is a pictorial record of the detector response as a function of elution volume or retention time, It consists of a series of peaks or bands.

#Id: 2782

#Unit 13. Methods in Biology

The retention time tR for each analyte has two components

 i - dead time, tM. &

ii - adjusted retention time, t’R.

#Id: 2783

#Unit 13. Methods in Biology

t’R is characteristic of the analyte and is the difference between the observed retention time and the dead time: