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#Unit 13. Methods in Biology
Oligonucleotide-directed mutagenesis.
This technique requires a knowledge of nucleotide sequence, since an oligonucleotide may then be synthesized with the base mutation.
Annealing of the oligonucleotide to complementary (except for the mutation) single-stranded DNA provides a primer for DNA polymerase to produce a new strand and thus incorporates the primer with the mutation.
