RPE65 is the enzyme in the pigment epithelial cells of the retina that catalyzes the isomerization of the intermediate esterified all-trans retinol to 11-cis-retinol.

TLS Online TPP Program

#Id: 6214

#Unit 12. Applied Biology

Adenoviral vectors

Adenoviruses are large linear double-stranded DNA viruses. Their large size enables them to accept large inserts. 

TLS Online TPP Program

#Id: 6215

#Unit 12. Applied Biology

These vectors are advantageous in that they can deliver many genome copies per host cell and so typically are associated with high expression. 

TLS Online TPP Program

#Id: 6216

#Unit 12. Applied Biology

Proteins encoded by early region (E1, E2, E3, E4) genes are involved in viral DNA replication, cell cycle modulation and defense system. The late region genes (L1, L2, L3, L4, L5) encodes the viral structural proteins.

TLS Online TPP Program

#Id: 6217

#Unit 12. Applied Biology

TLS Online TPP Program

#Id: 6218

#Unit 12. Applied Biology

The genetic expression from adenoviruses is transient, where the DNA is free within the host cell’s nucleus (episomal) where it can be transcribed, but upon cell division these genes will not be replicated.

TLS Online TPP Program

#Id: 6219

#Unit 12. Applied Biology

Third generation adenoviral Vectors also known as helper dependent adenovirus as they lack all the coding sequences and require helper virus which carries all the coding sequences needed for successful infection like viral DNA replication, viral assembly and infection of new cells etc.