Tools against infectious diseases

#Id: 2515

#Unit 13. Methods in Biology

Pulsed-Field Gel Electrophoresis involves electrophoresis in agarose, where two electric fi elds are applied alternately at different angles for defined time periods (e.g. 60 s)

#Id: 2516

#Unit 13. Methods in Biology

 PFGE has proved particularly useful in identifying the course of outbreaks of bacterial food-borne illness (e.g. Salmonella infections).

#Id: 2517

#Unit 13. Methods in Biology

DNA from Salmonella species, when digested with the restriction enzyme Xba I, gives around 15 fragments ranging from 25 kb to 680kb. This pattern of fragments, or ‘fingerprint’, is unique to that strain. If the same fingerprint is found from bacteria from other infected people, then it can be assumed that they were all infected from a common source which can be analyzed by PFGE

#Id: 2518

#Unit 13. Methods in Biology

Electrophoresis of normal RNA can be done in a 2% agarose gel in about 1 h while, Ribosomal RNAs (18 S and 28 S) are clearly resolved in 2.5–5% acrylamide gradient gel with an overnight run. 

#Id: 2519

#Unit 13. Methods in Biology

Capillary zone electrophoresis is the example of free solution electrophoresis with highest resolution using high voltage and using Electrophoretic mobility (EFM) & Electroendosmosis force (EEOF).

#Id: 2520

#Unit 13. Methods in Biology

Capillary zone electrophoresis can analyze hybridization probes, gene-cloning, DNA sequencing, polymerase chain reaction (PCR), HIV load, site-directed mutagenesis, & antisense therapeutics.