What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?

I. Transform bacteria with recombinant DNA molecule.

II. Cut the plasmid DNA using restriction enzymes.

III. Extract plasmid DNA from bacterial cells.

IV. Hydrogen-bond the plasmid DNA to non plasmid DNA fragments.

V. Use ligase to seal plasmid DNA to non plasmid DNA.

#Question id: 31338

#Unit 2. Cellular Organization

#Question id: 10221

#Unit 6. System Physiology – Plant

plastoquinone is one of the electron carriers between PSI and PSII, accumulates in the reduced state. Reduced plastoquinone accumulates when

a.) PSI is being activated more frequently than PSII

b.) PSII is being activated more frequently than PSI  

c.) LHCII is not phophorylated

d.) LHCII is phophorylated

Which of the following state will correct?

#Question id: 12373

#Unit 10. Ecological Principles

#Question id: 9196

#Unit 9. Diversity of Life Forms

If all individuals in the last remaining population of a particular frog species were all highly elated, which type of diversity would be of greatest concern when planning to prevent the species from going extinct?

#Question id: 8712

#Unit 9. Diversity of Life Forms

The lakes of northern Minnesota are home to many similar species of damselflies of the genus Enallagma. These species have apparently undergone speciation from ancestral stock since the last glacial retreat about 10 thousand years ago. Sequencing which of the following would probably be most useful in sorting out evolutionary relationships among these closely related species?