Many eukaryotic genes contain a large number of exons. Correct splicing of such genes requires that neighboring exons be ligated to one another; if they are not, exons will be left out. One early proposal suggested that the splicing machinery bound to a splice site at one end of an intron and scanned through the intron to find the splice site at the other end. Such a scanning mechanism would guarantee that an exon was never skipped. This hypothesis was tested with one minigene with a duplicated 3ʹ splice site. Find diagram of the products you expect from minigene if the splicing machinery binds to a 3ʹ splice site and scans toward a 5ʹ splice site.

#Question id: 13191

#Unit 10. Ecological Principles

#Question id: 1255

#Unit 4. Cell Communication and Cell Signaling

Following statements are regarding to the mechanism of action of cholera toxin.

A. cholera toxin is a homodimeric protein, target G proteins, interfering with normal signaling in host cells.

B. cholera toxin associates with a small G protein, known as ARF6.

C. association with ARF6 activates cholera toxin, which catalyzes the transfer of ADP-ribose from NAD+ to the critical Arg residue in the P loop of the alpha subunit of Gs.

D. ADP-ribosylation blocks the GEF activity of Gs and thereby renders Gs permanently inactive.

E. This results in continuous activation of the adenylyl cyclase of intestinal epithelial cells, chronically high cAMP, and active PKA dephosphorylates the CFTR Cl- channel and a sodium-proton exchanger in the intestinal epithelial cells.

Which of the following combination is correct?

#Question id: 10303

#Unit 6. System Physiology – Plant

During glycolysis, if radiolabelled the glucose at 3 or 4 carbon then what will be the correct prediction about radiolabelling in the cycle?

#Question id: 765

#Unit 1. Molecules and their Interaction Relevant to Biology

miRNA plays a role in transcriptional and post transcriptional regulation by which of the following

#Question id: 10930

#Unit 7. System Physiology – Animal

A 34-year-old man with schizophrenia has had chronic fatigue for 6 months. He has a good appetite, but has refused to eat vegetables for 1 year because he hears voices saying that vegetables are poisoned. His physical and neurological examinations are normal. His hemoglobin level is 9.1 g/dL, leukocyte count is 10,000/μl3, and MCV is 122. Which of the following is the most likely diagnosis?