What is order of steps to visualise Individual bands containing particular genes can be identified by in situ hybridization.

A. Target cells squashed on slide and freeze in dry ice

B. Wash with ethanol

C. Dip in agar solution

D. Denature DNA

E. Add radioactive probe

F. Wash off unreacted probe

G. Autoradiography

TLS Online TPP Program

#Question id: 18905

#Unit 13. Methods in Biology

A Vector is a DNA molecule that has the ability to

TLS Online TPP Program

#Question id: 18906

#Unit 13. Methods in Biology

A good vector must have the following properties.

a) It should be able to replicate autonomously.

b) A vector should be ideally less than 10 kb in size

c) The vector should be easy to isolate and purify.

d)  vector should have suitable marker genes that allow easy detection and/or selection of the transformed host cells.

e) it should a multiple cloning site

TLS Online TPP Program

#Question id: 18907

#Unit 13. Methods in Biology

When expression of the DNA insert is desired, the vector should contain at least suitable control elements, 

TLS Online TPP Program

#Question id: 18908

#Unit 13. Methods in Biology

pBR 322 has/have which of the following selection marker(s)?

TLS Online TPP Program

#Question id: 18909

#Unit 13. Methods in Biology

What is the compound X-gal?

TLS Online TPP Program

#Question id: 18910

#Unit 13. Methods in Biology

is a lactose analog which is broken down by enzyme beta-galactosidase to give a product deep blue in colour.