Cellular membranes are self-sealing— if they are punctured or disrupted mechanically, they quickly and automatically reseal. What properties of membranes are responsible for this important feature?

TLS Online TPP Program

#Question id: 12933

#Unit 13. Methods in Biology

Choose the correct order for the cloning process.

1. The production of cDNA is carried out using an enzyme termed Reverse transcriptase.

2. mRNA cannot be used directly in cloning.

3. It is possible to synthesis complementary DNA molecule to all the mRNA from the selected tissue.

4. The cDNA may be inserted into vectors and then cloned.

TLS Online TPP Program

#Question id: 12934

#Unit 13. Methods in Biology

Reverse transcriptase enzyme is isolated from____

TLS Online TPP Program

#Question id: 12935

#Unit 13. Methods in Biology

 The integrity of the RNA should always be checked by _____

TLS Online TPP Program

#Question id: 12936

#Unit 13. Methods in Biology

Adding small double-stranded molecules with one internal site for a restriction endonuclease,termed ______ to cDNA.

TLS Online TPP Program

#Question id: 12937

#Unit 13. Methods in Biology

Choose the incorrect statement about Yeast Artificial Chromosome vectors.

TLS Online TPP Program

#Question id: 12938

#Unit 13. Methods in Biology

What is the main advantage of using Yeast Artificial Chromosome based vector over other vectors?

1. It has ability to clone very large fragments of DNA.

2. YAC vectors do not contain origin of replication sites 

3. It has insert  range up to 2000 kb.

4. It help in genome mapping and sequencing projects.