TLS Online TPP Program

#Question id: 785


Several types of very short RNAs repress—or silence—expression of genes with homology to those short RNAs. They are produced from a variety of sources and direct the silencing of genes in the following different ways-

#Part-B Specialized Branches in Biotechnology
  1. Triggering destruction of the mRNA encoded by the target gene

  2. Inhibiting translation of the mRNA

  3. Inducing chromatin modifications within the target gene and thereby silencing its transcription

  4. All of the above

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TLS Online TPP Program

#Question id: 19887

#Part-A Aptitude & General Biotechnology

Paul Berg’s gene splicing experiment created the first rDNA molecules which was a

TLS Online TPP Program

#Question id: 19888

#Part-A Aptitude & General Biotechnology

There are some typical enzyme used in the manipulation of nucleic acid;

                 

                 ENZYMES

 

USE IN NUCLEIC ACID MANIPULATION

 

A) klenow

 

i) DNA-dependent RNA polymerase

 

B) T4 DNA polymerase

 

ii) Single-strand specific nuclease

 

C) Taq DNA polymerase

 

iii) DNA pol I lacks 5’→ 3’ exonuclease activity

 

D) T7 RNA polymerase

 

iv) Thermostable DNA polymerase used in PCR

 

E) S1 nuclease

 

v) Lack  5’→ 3’ exonuclease activity

TLS Online TPP Program

#Question id: 19929

#Part-B Specialized Branches in Biotechnology

he growth of an organism on glucose is described by the following Monod model parameters: μm = 0.5 h-1 and Ks =0.1 g.l-1, if the concentration of glucose in the feed is 10 g.l-1 and the dilution rate is set to 0.4 h-1, then the steady state concentration of glucose in the effluent will be

TLS Online TPP Program

#Question id: 19954

#Part-B Specialized Branches in Biotechnology

A continuous reactor at steady state contains 0.04 g.l-1 of biomass and 0.02 g.l-1 of phenol. The feed contained 0.1 g.l-1 of phenol. The biomass yield would be

TLS Online TPP Program

#Question id: 19955

#Part-B Specialized Branches in Biotechnology

A culture system with constant environmental conditions maintained through continual provision of nutrient and removal of wastes is called __________ culture system.

TLS Online TPP Program

#Question id: 19955

#Part-A Aptitude & General Biotechnology

A culture system with constant environmental conditions maintained through continual provision of nutrient and removal of wastes is called __________ culture system.