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TLS Online TPP Program

#Question id: 19293

Choose the incorrect statement for the preparation of genomic libraries.

#Part-A Aptitude & General Biotechnology
  1. The first step is the isolation of genomic DNA
  2. Physical damage to the DNA should be avoided
  3. If a nuclear DNA library is to be constructed, organelle DNA is to be removed
  4. For the construction of organelle library, organelle DNA is purified from the nuclear DNA
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TLS Online TPP Program

#Question id: 19844

#Part-A Aptitude & General Biotechnology

Match the following column, which correctly represent the qPCR done by three methods and measure such as;

                  COLUMN I

                    COLUMN II

A) SYBR green

i) Annealing

B) Taqman Assay

ii) Denaturation

C) Molecular beacon

iii) pH

 

iv) Amplification

 

v) Extension

TLS Online TPP Program

#Question id: 19845

#Part-A Aptitude & General Biotechnology

SYBR Green qPCR method is based on____I_______ with the _____II______ relationship.
TLS Online TPP Program

#Question id: 19845

#Part-A Aptitude & General Biotechnology

SYBR Green qPCR method is based on____I_______ with the _____II______ relationship.
TLS Online TPP Program

#Question id: 19846

#Part-A Aptitude & General Biotechnology

Variants of PCR has been developed on the basis of___
TLS Online TPP Program

#Question id: 19865

#Part-A Aptitude & General Biotechnology

Match the following activity of variants in Column I with their variants of PCR in Column II given below;

                     COLUMN I

                        COLUMN II

 

A) Enhance efficiency

 

i) Multiplex PCR

 

B) Sequence specific amplification

 

ii) Hot-start PCR

 

C) Mutational analysis

 

iii) Megaprimer PCR

 

 

iv) qPCR with SYBR

 

 

v) AS-PCR

 

 

vi) RLM-RACE

TLS Online TPP Program

#Question id: 19866

#Part-A Aptitude & General Biotechnology

Which of the following variants of PCR has been devised to increase the specificity of PCR without lowering the efficiency by variation annealing temperature?