For analyzing DNA sequences, two techniques have been developed, one is _________ method frequently termed_________, involving the enzymatic synthesis of DNA and stopping the synthesis at specific nucleotides. While another technique, the __________ method of DNA sequencing developed by ___________ is often used for sequencing small fragments of DNA.

TLS Online TPP Program

#Question id: 19789

#Part-B Specialized Branches in Biotechnology

How to determine the size of PCR product?

TLS Online TPP Program

#Question id: 19790

#Part-A Aptitude & General Biotechnology

What will be the major difference between the PCR and replication is?

TLS Online TPP Program

#Question id: 19790

#Part-A Aptitude & General Biotechnology

What will be the major difference between the PCR and replication is?

TLS Online TPP Program

#Question id: 19791

#Part-A Aptitude & General Biotechnology

Which of the following statements about polymerase chain reaction is correct?

a) PCR denaturation temperature calculated by using Y = X (1+E)n

b) Size of the primer negatively affects the efficiency of PCR

c) Standard PCR doesn’t efficiently amplify sequence much longer than about 5kb.

d) Primer annealing of PCR at 550C for 1.5 min.

e) Time taken for each cycle of PCR considerably longer than 3 min.

TLS Online TPP Program

#Question id: 19791

#Part-A Aptitude & General Biotechnology

Which of the following statements about polymerase chain reaction is correct?

a) PCR denaturation temperature calculated by using Y = X (1+E)n

b) Size of the primer negatively affects the efficiency of PCR

c) Standard PCR doesn’t efficiently amplify sequence much longer than about 5kb.

d) Primer annealing of PCR at 550C for 1.5 min.

e) Time taken for each cycle of PCR considerably longer than 3 min.

TLS Online TPP Program

#Question id: 19840

#Part-A Aptitude & General Biotechnology

Match the following source of thermostable DNA polymerase with proofreading (3’−5’ exonuclease) activity;

DNA Polymerase	Sources
A- Tma	i) Pyrococcus furiosus
B- Deep Vent™	ii) Pyrococcus woesi
C- Tli	iii) Thermotoga maritima
D- Pfu	iv) Pyrococcus sp.
E- Pwo	v) Thermococcus litoralis