Correct statements about RAPD’s

A. RAPD polymorphism is detected by using oligonucleotides usually more than 10 bases long of random sequences as primers in a reaction.

B. In a strain which has in genomic DNA sequences complementary to the primer oligonucleotide, PCR products will be detected in the gel,

C. Typical RAPD markers show limited variation between parents, especially in naturally inbreeding species.

D. RAPDs are more sensitive than RFLPs to experimental conditions making them more difficult to be consistent and reproducible.

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#Question id: 18844

#Part-A Aptitude & General Biotechnology

In the extraction of DNA from cell with to remove the high protein content from the cell , before phenol treatment which of the following treatment is done 

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#Question id: 18845

#Part-A Aptitude & General Biotechnology

The action of EDTA in EDTA-lysozyme treatment in bacterial DNA extraction is

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#Question id: 18846

#Part-A Aptitude & General Biotechnology

For an application where you require a sample of your target protein at high purity, what would be a good purification strategy? Assume that your starting point is E. coli cells in which the target protein fused to an affinity tag has been over-expressed.

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#Question id: 18847

#Part-A Aptitude & General Biotechnology

You know that the protein you want to purify from a natural source forms a multimer with multiple sub-units giving a molecular weight in solution much bigger than visualised denatured on SDS-PAGE. There is only a small amount of the target protein in the total protein sample. Which of the following is an appropriate purification strategy?

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#Question id: 18848

#Part-A Aptitude & General Biotechnology

You find that your protein sample loses activity during storage. What can you do about this?

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#Question id: 18849

#Part-A Aptitude & General Biotechnology

Which of these techniques is often considered a suitable "polishing" step in a protein purification strategy?