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Nonpolar amino acids have three ionisations zones
1. 1--------pK1 – charge +1
2. pK1---------pK2 charge 0
3. pK2---------14 charge -1

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Anammox is anaerobic production of molecular nitrogen from NH4 +and NO2–

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N2 constitutes about 80% of the earth’s atmosphere, but the stability of its triple bond renders it inert and metabolically inaccessible to most organisms.

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