Nutrient medium is to be heated from 10⁰C to 28⁰C is a single-pass countercurrent shell-and-tube heat exchanger before being pumped into a fed-batch fermenter. Medium passes through the tubes of the exchanger; the shell-side fluid is water which enters with flow rate 3 x 10^4 kg h^- 1 and temperature 60⁰C pre-heated medium is required at a rate of 50 m^3 h^-1. The density, viscosity and heat capacity of the medium are the same as water; the thermal conductivity of the medium is 0.54 Wm^- 1 ⁰C^ - 1. It is proposed to use 30 steel tubes with inner diameter 5 cm; the tubes will be arranged in line. The pipe wall is 5-mm thick; the thermal conductivity of the metal is 50 Wm^- 1 ⁰C^ - 1. The maximum linear shell-side fluid velocity is estimated as 0.15 m s^-1.  Determine the heat-transfer area. ____________

TLS Online TPP Program

#Question id: 14801

#Section 5: Bioprocess Engineering and Process Biotechnology

In a chemostat operating under steady state, a bacterial culture can be grown at dilution rate higher than maximum rate by

TLS Online TPP Program

#Question id: 14802

#Section 5: Bioprocess Engineering and Process Biotechnology

Column I                                                                                  column II

A. Hydrophobic interaction chromatography (HIC)          1. gradient stepwise

B. Ion exchange chromatography                                       2. isocratic

C. Gel filtration chromatography                                         3. stepwise

D. Affinity chromatography                                                  4.  Gradient stepwise

A       B       C      D

1        2       3       4

3        1       2       4

1       4        2       3

1       3        4       2

TLS Online TPP Program

#Question id: 14803

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The percentage SIMILARITIES and IDENTITIES, respectively, between the two peptide sequences given below will be ______% and _____%.

Peptide I :

Ala-Ala-Arg-Arg-Gln-Trp-Leu-Thr-Phe-Thr-Lys-Ile-Met-Ser-Glu

Peptide II:

Ala-Ala-Arg-Glu-Gln-Tyr-Ile-Ser-Phe-Thr-Lys-Ile-Met-Arg-Asp

TLS Online TPP Program

#Question id: 14804

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Calculate the percentage sequence identity for the pairwise alignment given below: 

                       H E L L O _

                       Y E L L O W

TLS Online TPP Program

#Question id: 14844

#Section 1: Engineering Mathematics

An urn contains 25 balls numbered 1 through 25. Two balls are drawn from the urn with replacement. The probability of getting at least one odd is

TLS Online TPP Program

#Question id: 14845

#Section 1: Engineering Mathematics

Four persons are chosen at random from a group containing 3 men, 2 women and 4 children. The chance that exactly 2 of them will be children is