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TLS Online TPP Program

#Question id: 14253

Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  Determine the volume of the packed bed for 95% glucose conversion. Bed diameter is D0 = 0.2 m. Neglect the growth of the hybridomas and assume first order kinetics. 

#Section 5: Bioprocess Engineering and Process Biotechnology
  1. 0.029m^3           
  2. 0.52m^3 
  3. 0.84m^3 
  4. 0.098m^3   
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TLS Online TPP Program

#Question id: 15504

#Section 4: Fundamentals of Biological Engineering

Saccharomyces cereviseae produces ethanol by fermentation. The theoretical yield of ethanol from 2.5 g of glucose is
TLS Online TPP Program

#Question id: 15505

#Section 4: Fundamentals of Biological Engineering

The empirical formula for biomass of an unknown organism is CH1.8O0.5N0.2. To grow this organism, ethanol (C2H5OH) and ammonia are used as carbon and nitrogen
sources, respectively. Assume no product formation other than biomass. To produce 1 mol of biomass from 1 mol of ethanol, the number of moles of oxygen required will be
TLS Online TPP Program

#Question id: 15506

#Section 5: Bioprocess Engineering and Process Biotechnology

For the reaction given by 2NO + O2 → 2NO2, The rate equation is:
Rate = k[NO]2[O2]
Find the overall order of the reaction and the units of the rate constant.
TLS Online TPP Program

#Question id: 15507

#Section 5: Bioprocess Engineering and Process Biotechnology

The rate constant of a reaction is k=3.28×10-4 s-1. Find the order of the reaction.
TLS Online TPP Program

#Question id: 15508

#Section 5: Bioprocess Engineering and Process Biotechnology

The reaction rate is defined as the rate at which the concentration of the reactants ___with time or the concentration of products ___ with time.
TLS Online TPP Program

#Question id: 15509

#Section 5: Bioprocess Engineering and Process Biotechnology

If the zero order reaction takes 100 min for 50% completion, how much time will it take for the 75% completion of the reaction?