Practice Questions

TLS Online TPP Program

#Question id: 14309

Glucose is converted to ethanol by immobilized yeast cells entrapped in gel beads. The specific rate of ethanol production is: q_P = 0.2 g ethanol/g-cell-h. The effectiveness factor for an average bead is 0.8. Each bead contains 50 g/L of cells. The voids volume in the column is 40%. Assume growth is negligible (all glucose is converted into ethanol). The feed flow rate is F = 400 l/h and glucose concentration in the feed is S_0i = 150 g glucose/l. The diameter of the column is 1 m and the yield coefficient is about 0.49 g ethanol/g glucose. The column height is 4 m. What is the glucose conversion at the exit of the column?

#Section 5: Bioprocess Engineering and Process Biotechnology

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#Question id: 13076

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i. Large molecular weight genomic DNA is isolated from several strains or related species;

ii. The fragments in these digests are separated through electrophoresis

iii. These 'DNAs are then digested with a selected restriction enzyme

iv. Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v. The resulting gel lanes are transferred and fixed to a suitable solid support and

vi. The free probes are removed

vii. The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

TLS Online TPP Program

#Question id: 13077

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The DNA probes for RFLP may NOT be obtained from

TLS Online TPP Program