Production of recombinant protein by a geneticallyengineered strain of Escherichia coli is proportional to cell growth. Ammonia is used as nitrogen source for aerobic respiration of glucose. The recombinant protein has an overall formula CHI.5500.31N0.25. The yield ofbiomass from glucose is measured at 0.48 g g-1; the yield of recombinant protein from glucose is about 20% that for cells. If the biomass yield remains at 0.48 g g- l, how much change in ammonia requirement for wild-type E. coliunable to synthesise recombinant protein?

#Question id: 5241

#Section 3: Genetics, Cellular and Molecular Biology

Genes a, b, and c are widely spaced in the bacterial genome. Transducing phage from an a+ b+ c+ bacterium were used to infect a culture of a- b- c- cells, and b+ transductants were selected. Which of the following best describes the predicted genotypes of these transductants?

#Question id: 5242

#Section 3: Genetics, Cellular and Molecular Biology

Auxotrohic bacteria plating on different medium, it is observed that colonies grow on minimal plus threonine only, no colony on minimal plus leucine and no colonies grow on minimal medium cystein only. What are the actual genotypes of auxotroph?

#Question id: 5243

#Section 3: Genetics, Cellular and Molecular Biology

Auxotroph culture (His- and Lac-) culture of E. coli was mutagenised. On what media would one spread the mutagenised cells to select prototroph for both His+  and Lac+ cells?

#Question id: 5244

#Section 3: Genetics, Cellular and Molecular Biology

Transformation can used to map bacterial gene, which of the following statement is correct?

#Question id: 5244

#Section 3: Genetics, Cellular and Molecular Biology

Transformation can used to map bacterial gene, which of the following statement is correct?

#Question id: 5245

#Section 3: Genetics, Cellular and Molecular Biology

Which method of gene transfer involves direct contact between the bacteria?