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TLS Online TPP Program

#Question id: 71

Which of the following is not the priority rule for R, S Configuration?

#Section 2: General Biology

  1. If the four atoms attached to the chiral center are all different, priority depends on atomic number, with the atom of lower atomic number getting lower priority.

  2. If the two atoms attached to chiral centers are same, the atoms attached to each of these first atoms are compared

  3. When there is a double bond or triple bond, both atoms are considered to be duplicated or triplicated

  4. If the four atoms attached to the chiral center are all different, priority depends on atomic number, with the atom of higher atomic number getting lower priority.

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TLS Online TPP Program

#Question id: 13032

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

In SDS-PAGE, protein sample is first treated with detergent sodium dodecyl sulfate (SDS), in order to
TLS Online TPP Program

#Question id: 13033

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The sample is then fractionated by electrophoresis through a _______. As all the proteins now have an _______ to mass ratio, they are separated on the basis of their _____.
TLS Online TPP Program

#Question id: 13034

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which the following statements is/are true about the stacking gel?
i. The stacking gel solution contains acrylamide.
ii. It is on the top of the separating gel.
iii. It is used to dilute the protein sample before it enters the main separating gel.
iv. It is into this gel that the wells are formed and the proteins loaded.
TLS Online TPP Program

#Question id: 13035

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Each band on the gel represents a different protein (or protein subunit); _____ proteins move through the gel more _____ than_____ proteins and therefore are found nearer the _____ of the gel.
TLS Online TPP Program

#Question id: 13036

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

In isoelectric focusing the protein will move until
TLS Online TPP Program

#Question id: 13037

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following proteins will be most far away from the positively charged electrode during isoelectric focusing in a buffer with pH of 5?