Practice Questions

TLS Online TPP Program

#Question id: 1967

Which of the following is the most likely reason that ancient peoples sought to identify inflammation?

#Section 2: General Biology

Seeing such signs would be cause for their seeking out a healer in their community.
Presence of the signs of inflammation in a patient could be a condemnation of the healer.
The ancients probably knew of plant derivatives that could reduce the pain of inflammation.
If these signs were present, they would know that healing was taking place; otherwise the patient would likely die.

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TLS Online TPP Program

#Question id: 14250

#Section 5: Bioprocess Engineering and Process Biotechnology

A stirred-tank reactor is to be scaled down from 10 m3 to 0.1 m3. The dimensions of the large tank are: Dt = 2 m; Di = 0.5 m; N = 100 rpm. What would be the required rotational speed of the impeller in the small tank if impeller Re number is constant?

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TLS Online TPP Program

#Question id: 14251

#Section 5: Bioprocess Engineering and Process Biotechnology

Yeast cells are recovered from a fermentation broth by using a tubular centrifuge. Sixty percent (60%) of the cells are recovered at a flow rate of 12 l/min with a rotational speed of 4000 rpm. Recovery is inversely proportional to flow rate. a. To increase the recovery of cells to 95% at the same flow rate, what should be the rpm of the centrifuge?

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TLS Online TPP Program

#Question id: 14252

#Section 5: Bioprocess Engineering and Process Biotechnology

Yeast cells are recovered from a fermentation broth by using a tubular centrifuge. Sixty percent (60%) of the cells are recovered at a flow rate of 12 l/min with a rotational speed of 4000 rpm. Recovery is inversely proportional to flow rate. At a constant rpm of 4000 rpm, what should be the flow rate to result in 95% cell recovery?

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TLS Online TPP Program

#Question id: 14253

#Section 5: Bioprocess Engineering and Process Biotechnology

Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient. Determine the volume of the packed bed for 95% glucose conversion. Bed diameter is D0 = 0.2 m. Neglect the growth of the hybridomas and assume first order kinetics.

TLS Online TPP Program

#Question id: 14254

#Section 5: Bioprocess Engineering and Process Biotechnology

Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient. Determine the height of the packed bed for 95% glucose conversion. Bed diameter is D0 = 0.2 m. Neglect the growth of the hybridomas and assume first order kinetics.

TLS Online TPP Program

#Question id: 14255

#Section 5: Bioprocess Engineering and Process Biotechnology

Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient. If Yp/s is 4 mg Mab/g glu, determine the effluent Mab concentration of the system?