Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

 Which of the following is true for traditional and real time PCR?

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#Question id: 27236

#Section 3: Genetics, Cellular and Molecular Biology

Group of adjacent nucleotides are joined by ____________

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#Question id: 15941

#Section 1: Engineering Mathematics

The factorized form of the following determinant is:

 

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#Question id: 12939

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Choose the incorrect statement for the production of site-directed mutations.

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#Question id: 17201

#Section 1: Engineering Mathematics

Company A produces 10% defective products, Company B produces 20% defective products and C produces 5% defective products. If choosing a company is an equally likely event, then find the probability that the product chosen is defective...........

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#Question id: 14915

#Section 1: Engineering Mathematics

For what values of k, with  and k > 0 does  converges