Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

 Which of the following is true for traditional and real time PCR?

#Question id: 3754

#Section 3: Genetics, Cellular and Molecular Biology

Thymine makes up 28% of the nucleotides in a sample of DNA from an organism. Approximately what percentage of the nucleotides in this sample will be guanine?

#Question id: 3755

#Section 3: Genetics, Cellular and Molecular Biology

In E. coli, there is a mutation in a gene called dnaB that alters the helicase that normally acts at the origin of replication. Which of the following events would you expect to occur as a result of this mutation?

#Question id: 3756

#Section 3: Genetics, Cellular and Molecular Biology

 In E. coli, which enzyme catalyzes the elongation of a new DNA strand in the 5' → 3' direction?

#Question id: 3757

#Section 3: Genetics, Cellular and Molecular Biology

Which of the following characteristics of eukaryotic telomeres cause them to replicate differently than the rest of the chromosome?

#Question id: 3758

#Section 3: Genetics, Cellular and Molecular Biology

 At a specific area of a chromosome, the sequence of nucleotides below is present where the chain opens to form a replication fork:

3' C C T A G G C T G C A A T C C 5'

An RNA primer is formed starting at the underlined T (T) of the template. Which of the following represents the primer sequence?

#Question id: 3759

#Section 3: Genetics, Cellular and Molecular Biology

In E. coli, to repair a thymine dimer by nucleotide excision repair, in which order do the necessary enzymes act?