Match the following:

A. DNA polymerases              i. Alkaline phosphatase        a.RNA-dependent DNA polymerase, used in cDNA synthesis

B. Reverse transcriptase        ii. T4 DNA ligase                    b. Thermostable DNA polymerase used in PCR

C. Ligases                                 iii.  AMV-RT                            c. Removes 5′-phosphates from DNA and RNA

D. Phosphatases                     iv. Taq DNA pol                      d. Links 5′-phosphate and 3′-hydroxyl ends via phosphodiester bond

TLS Online TPP Program

#Question id: 12922

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

 Match the following:-

1. Ligase chain reaction.              i. Mutation detection

2. Nucleic acid sequence based   amplification.                            ii.Non isothermal                   

3. Loop mediated isothermal amplification.                      iii. Viral detection

4. Branched DNA amplification.   iv.parasitic disease

TLS Online TPP Program

#Question id: 12923

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Name the technology which is used as a means of identifying the initial concentrations of DNA or cDNA template used.

TLS Online TPP Program

#Question id: 12924

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

In order to quantify unknown DNA templates, a standard dilution is prepared using DNA of known concentration. As the DNA accumulates during the early exponential phase of the reaction,an arbitrary point is taken where each of the diluted DNA samples cross. This is termed as_____

TLS Online TPP Program

#Question id: 12925

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Another name of TaqMan assay is ____

TLS Online TPP Program

#Question id: 12926

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

PCR is undertaken with a fluorescence reporter / quencher pair called______

TLS Online TPP Program

#Question id: 12926

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

PCR is undertaken with a fluorescence reporter / quencher pair called______