TLS Online TPP Program

#Question id: 12908


Which statement is incorrect about CRISPR?

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
  1. It  was first identified as an adaptive immunity pathway in prokaryotes providing resistance to bacteriophage and analogous to the RNA interference process found in eukaryotes. 
  2. It does not utilize double-stranded sequence-specific breaks and does not  repair using HDR via homologous recombination.
  3. The system consists of two parts, a short synthetic guide RNA ( gRNA) and a nonspecific double-stranded endonuclease termed Cas9. 
  4. It is also possible to program Cas9 with multiple guide RNAs to allow multiplex site-specific editing for designing large deletions, inversions and translocations.