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TLS Online TPP Program

#Question id: 12908

Which statement is incorrect about CRISPR?

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
  1. It  was first identified as an adaptive immunity pathway in prokaryotes providing resistance to bacteriophage and analogous to the RNA interference process found in eukaryotes. 
  2. It does not utilize double-stranded sequence-specific breaks and does not  repair using HDR via homologous recombination.
  3. The system consists of two parts, a short synthetic guide RNA ( gRNA) and a nonspecific double-stranded endonuclease termed Cas9. 
  4. It is also possible to program Cas9 with multiple guide RNAs to allow multiplex site-specific editing for designing large deletions, inversions and translocations.
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TLS Online TPP Program

#Question id: 15491

#Section 4: Fundamentals of Biological Engineering

The respiratory coefficient for the reaction    is defined as
   
TLS Online TPP Program

#Question id: 15492

#Section 4: Fundamentals of Biological Engineering

Assume that experimental measurements for a certain organism have shown that cells can convert two-thirds (wt/wt) of the substrate carbon to biomass carbon. Calculate the stoichiometric coefficients for the following biological reactions:
 Hexadecane: C16H34 + aO2 + bNH3 c(C4.4H7.3N0.86O1.2) + dH2O + eCO2
TLS Online TPP Program

#Question id: 15493

#Section 4: Fundamentals of Biological Engineering

Assume that experimental measurements for a certain organism have shown that cells can convert two-thirds (wt/wt) of the substrate carbon to biomass carbon. Calculate the stoichiometric coefficients for the following biological reactions:

Glucose: C6H12O6 + aO2 + bNH3 c(C4.4H7.3N0.86O1.2) + dH2O + eCO2
TLS Online TPP Program

#Question id: 15494

#Section 4: Fundamentals of Biological Engineering

Assuming that cells convert two-thirds (w/w) of the substrate to biomass, the stoichiometric coefficient (1 only) for glucose utilisation for the following reaction is
TLS Online TPP Program

#Question id: 15495

#Section 4: Fundamentals of Biological Engineering

Calculate the degree of reduction for following substrates:
A) Methane
B) Glucose
C) Ethanol
TLS Online TPP Program

#Question id: 15496

#Section 4: Fundamentals of Biological Engineering

S. cerevisiae produces ethanol at a yield of 0.5 g/g glucose. The strain ferments 20g/l glucose in 24 hours. Calculate productivity of ethanol in this fermentation