What is -40°F in degrees centigrade? degrees Rankine? kelvin?

TLS Online TPP Program

#Question id: 3617

#Section 3: Genetics, Cellular and Molecular Biology

Alternate forms of a gene is called

TLS Online TPP Program

#Question id: 14238

#Section 5: Bioprocess Engineering and Process Biotechnology

A genetically-engineered strain of yeast is cultured in a bioreactor at 30⁰C for production of heterologous protein. The oxygen requirement is 80 mmol 1-1 h-I; the critical oxygen concentration is 0.004 mM. The solubility of oxygen in the fermentation broth is estimated to be 10% lower than in water due to solute effects. What mass-transfer coefficient is required if pure oxygen is used instead of air?                       

TLS Online TPP Program

#Question id: 13088

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 1’s plasmid is;

TLS Online TPP Program

#Question id: 14387

#Section 1: Engineering Mathematics

Given that A = [aij] is a square matrix of order 3x3 and IAI = -7, then the value of where Aij denotes the cofactor of element aij is 

TLS Online TPP Program

#Question id: 12910

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the following:

A. RNA analysis               i. Sexing or cell mutation sites               a. Expressed Sequence tag

B. Forensic science           ii. mRNA analysis                                     b.  Analysis of DNA from blood

C. Gene discovery              iii. DNA profiling                                       c. Active latent viral infections

D. Single-cell analysis     iv.  RT–PCR                                             d. Sex determination of unborn