Glycine & Proline are relatively polar considering negative hydropathy index (-0.4 & -1.6) comparing to other nonpolar amino acids.

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#XL - Q Biochemistry

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#XL - Q Biochemistry

The dependent variable is the rate of the reaction. 

The independent variables are the concentration of enzyme, the concentration of the substrate, the temperature, and the addition of an inhibitor

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If the concentration of the enzyme or substrate is increased, the rate of the reaction will be increased as well. 

If the temperature of the enzymes surrounding environment is increased, then the rate of the reaction will also be increased. 

If the substrate concentration is increased then the rate of the reaction will increase. 

If an inhibitor is added then the rate of the reaction will be decreased significantly

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#XL - Q Biochemistry

One drawback from the Eadie–Hofstee approach is that neither ordinate nor abscissa represent independent variables: both are dependent on reaction rate. Thus any experimental error will be present in both axes.

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The abscissa refers to the (x) coordinate and the ordinate refers to the (y) coordinate of a standard two-dimensional graph.

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#XL - Q Biochemistry

Irreversible inhibitors, such as the organophosphorus and organomercury compounds, cyanide, carbon monoxide and hydrogen sulphide, combine with the enzyme to form a covalent bond.