Matrix-targeting sequences

Located at the N-terminus

Usually 20–50 amino acids in length

Rich in hydrophobic amino acids, positively charged basic amino acids (arginine & lysine), and hydroxylated ones (serine and threonine)

Amphipathicity of matrix-targeting sequences is critical to their function

TLS Online TPP Program

#Id: 7914

#XL - Q Biochemistry

Matrix-targeting sequences

Located at the N-terminus

Usually 20–50 amino acids in length

Rich in hydrophobic amino acids, positively charged basic amino acids (arginine & lysine), and hydroxylated ones (serine and threonine)

Amphipathicity of matrix-targeting sequences is critical to their function

TLS Online TPP Program

#Id: 4932

#XL - Q Biochemistry

A non-competitive inhibitor can combine with an enzyme molecule to produce a dead-end complex.

TLS Online TPP Program

#Id: 4938

#XL - Q Biochemistry

TLS Online TPP Program

#Id: 8302

#XL - Q Biochemistry

TLS Online TPP Program

#Id: 8541

#XL - Q Biochemistry

The dependent variable is the rate of the reaction. 

The independent variables are the concentration of enzyme, the concentration of the substrate, the temperature, and the addition of an inhibitor

TLS Online TPP Program

#Id: 8542

#XL - Q Biochemistry

If the concentration of the enzyme or substrate is increased, the rate of the reaction will be increased as well. 

If the temperature of the enzymes surrounding environment is increased, then the rate of the reaction will also be increased. 

If the substrate concentration is increased then the rate of the reaction will increase. 

If an inhibitor is added then the rate of the reaction will be decreased significantly

TLS Online TPP Program

#Id: 8543

#XL - Q Biochemistry

One drawback from the Eadie–Hofstee approach is that neither ordinate nor abscissa represent independent variables: both are dependent on reaction rate. Thus any experimental error will be present in both axes.