After assembly of the matrix protein-Pex5-Pex14 complex, the matrix protein dissociates from Pex5 and is released into the peroxisomal matrix.

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The oxaloacetate is converted to PEP by phosphoenolpyruvate carboxykinase. This Mg2+ dependent reaction requires GTP as the phosphoryl group donor.

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Transketolase requires the cofactor thiamine pyrophosphate (TPP)

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Acetyl-CoA and NADH are potent allosteric inhibitors of the enzyme. 

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Acetyl-CoA inhibits the transacetylase component whereas NADH inhibits the dihydrolipoyl dehydrogenase component. 

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The reaction catalyzed by citrate synthase is essentially a Claisen condensation, involving a thioester (acetyl-CoA) and a ketone (oxaloacetate)

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Eukaryotic cells have two isozymes of aconitase

• The mitochondrial isozyme (convert citrate to isocitrate in the citric acid cycle)

• The cytosolic isozyme