#Question id: 5281

In the mating Hfr Strs met+ his+ leu+ trp+ Ala+   X F- Strres met- his- leu- trp- Ala-. At regular interval time, the mating cells are interrupted and the cell suspensions plated on four different growth media with streptomycin. The amino acids in the growth media and the number of colonies observed on each are as follows:

Interrupted time	Composition of media					Growth
	His	Leu	Ala	Trp	Met
2   min	+	-	+	+	+	Yes
10 min	+	-	-	+	+	Yes
12 min	+	-	-	+	-	No
15 min	+	-	-	-	+	Yes
20 min	-	-	-	-	+	Yes

What is the order of transfer of the genes?