Nurturing Life Sciences
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#Unit 3. Fundamental Processes
Hydrolysis of pyrophosphate makes phosphodiester bond a highly favorable reaction with a DG of –7 kcal/mol, which corresponds to an equilibrium constant (Keq) of 105.
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A:T and G:C base pairs have same geometry that’s why DNA polymerase has only one catalytic site for polymerization
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Incorrect base pairing leads to dramatically lower rates of nucleotide addition called as kinetic proofreading
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A common method to monitor synthesis of new DNA is Incorporation Assays.
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rNTPs are present at approximately 10-fold higher concentration in the cell, they are incorporated at a rate that is more than 1000-fold lower than dNTPs.
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The palm domain is composed of a b sheet and contains the primary elements of the catalytic site.