Nurturing Life Sciences
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#Unit 13. Methods in Biology
Linkers are fused with blunt-ended DNA fragments; cleavage of the linker with the appropriate restriction enzyme creates suitable cohesive protruding ends.
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Linkers have the following two applications: creation of cohesive 'ends
on blunt~ended DNA fragments, and
on fragments having unmatched or undefined sequences in their protruding ends.
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Examples of adapters. An adapter used to create BamHI sticky ends at the blunt ends of a DNA insert. A conversion adapter produced by associating two oligonucleotides, each having a different recognition sequence at its 5'~end but a complementary sequence for base pairing at the 3 '-end.
Examples of adapters.
An adapter used to create BamHI sticky ends at the blunt ends of a DNA insert.
A conversion adapter produced by associating two oligonucleotides, each having a different recognition sequence at its 5'~end but a complementary sequence for base pairing at the 3 '-end.
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Adapters are short, chemically synthesized DNA double strands, which already have one or both sticky ends.
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A gel mobility shift assay detects interaction between a protein and DNA by the reduction of the electrophoretic mobility of a small DNA that occurs on binding to a protein.
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A gel mobility shift assay, is a method for detecting DNA–protein interaction relies on the fact that a small DNA has a much higher mobility in gel electrophoresis than the same DNA does when it is bound to a protein.
